Name: SIST EN ISO 11701:2010 — Vegetable fats and oils - Determination of phospholipids in lecithins by high performance liquid chromatography (HPLC) using a light scattering detector (ISO 11701:2009)
SKU: SIST EN ISO 11701:2010
Price: 65.56 EUR
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Overview

SIST EN ISO 11701:2010 is an internationally recognized standard developed by CEN and ISO for the quantitative determination of phospholipid content in lecithins derived from vegetable fats and oils. The standard specifies a validated method that uses high performance liquid chromatography (HPLC) with a diol column and a light-scattering detector. This analytical approach ensures accurate identification and quantification of individual phospholipids, supporting quality control and compliance in the food, feed, and lecithin processing industries.

Keywords: vegetable lecithins, phospholipid determination, HPLC, light-scattering detector, vegetable oils, lecithin analysis, standard methods

Key Topics

Scope and Applicability
- Applies to crude, oil-containing lecithins, oil-free lecithins, and lecithin fractions from vegetable sources.
- Not suitable for animal lecithins, ruminant lecithins, or enzymatically hydrolysed lecithins due to insufficient peak separation for certain lysophospholipids.
Principle of Method
- Phospholipids in lecithins are separated on a diol HPLC column.
- Quantification is achieved using a calibrated light-scattering detector and certified reference mixtures.
Test Sample Preparation
- Importance of representative, homogenized samples.
- Samples are dissolved in specific solvent mixtures prior to analysis.
Precision and Repeatability
- Method precision is defined through international interlaboratory tests.
- Parameters for repeatability and reproducibility ensure reliability across laboratories.

Applications

Quality Control in Lecithin Production
- Ensures accurate phospholipid profiling in food-grade and technical-grade lecithins.
- Helps manufacturers verify that lecithin products meet regulatory and customer specifications.
Food and Feed Industry
- Useful for verifying the composition of lecithins used as emulsifiers in processed foods.
- Enables producers to standardize product functionality and performance.
Research and Development
- Supports technical research in the development of new lecithin fractions or applications.
- Facilitates investigation of phospholipid composition for nutritional labeling or functional food innovation.
Supply Chain Compliance
- Assists in certifying the authenticity and purity of vegetable lecithin fractions supplied to various industries.
- Meets international requirements for consistent product analysis and reporting.

Related Standards

ISO 661: Animal and Vegetable Fats and Oils - Preparation of Test Sample
- Provides guidance on test sample preparation, an essential pre-requisite for reliable HPLC analysis.
ISO 5555: Animal and Vegetable Fats and Oils - Sampling
- Establishes standardized procedures for sample collection, crucial for representative testing.
ISO 5725 Series: Accuracy (Trueness and Precision) of Measurement Methods and Results
- Defines methodology for assessing repeatability and reproducibility of analytical methods.
ISO 1042: Laboratory Glassware - One-mark Volumetric Flasks
- Specifies requirements for volumetric equipment used in sample and standard solution preparation.

Practical Value

Adhering to SIST EN ISO 11701:2010 provides the following practical advantages:

Assures Global Acceptance: Use of a harmonized, internationally recognized test provides credibility in global supply chains.
Supports Regulatory Compliance: Provides a scientific basis for compliance with food safety and ingredients regulations.
Enhances Product Consistency: Detailed phospholipid profiling helps maintain product quality and consistency, key for industrial applications.
Reduces Analytical Uncertainty: Standardized methodology minimizes variability and sources of error in phospholipid determination.

By implementing SIST EN ISO 11701:2010, laboratories and industry stakeholders ensure that the determination of phospholipids in vegetable lecithins is accurate, repeatable, and compliant with international best practices.

SIST EN ISO 11701:2010 PDF

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Abstract

Overview

Key Topics

Applications

Related Standards

Practical Value

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