Overview
SIST EN ISO 12193:2004 is an international standard developed by the International Organization for Standardization (ISO) and adopted by the European Committee for Standardization (CEN). It specifies a precise analytical method for determining trace levels of lead (Pb) in all types of crude and refined edible animal and vegetable fats and oils. The method utilizes direct graphite furnace atomic absorption spectroscopy (GFAAS), a sensitive technique capable of detecting lead concentrations as low as 0.001 mg/kg.
This standard is essential for ensuring the safety and quality of edible fats and oils by monitoring potential lead contamination, which is critical given lead’s toxicological risks to human health.
Key Topics
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Scope and Purpose
SIST EN ISO 12193:2004 defines a standardized approach to quantify trace amounts of lead in edible fats and oils, with a detection limit greater than 0.001 mg/kg, applicable to both crude and refined products.
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Analytical Principle
The method involves atomizing the sample within a graphite furnace, coupled to an atomic absorption spectrometer. Absorbance measurements at a wavelength of 283.3 nm allow for precise lead quantification.
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Sample Preparation and Reagents
Samples and calibration standards are mixed with a matrix modifier solution (2% lecithin in cyclohexane) to enhance accuracy. Blank oil with minimal lead content (