SIST EN ISO 14902:2002 PDF

Name: SIST EN ISO 14902:2002 — Animal feeding stuffs - Determination of trypsin inhibitor activity of soya products (ISO 14902:2001)
SKU: SIST EN ISO 14902:2002
Price: 60.09 EUR
Availability: InStock

Animal feeding stuffs - Determination of trypsin inhibitor activity of soya products (ISO 14902:2001)

Название (английский):

SIST EN ISO 14902:2002

Animal feeding stuffs - Determination of trypsin inhibitor activity of soya products (ISO 14902:2001)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 13
Дата публикации:: 1 июня 2002 г.
ICS:: 65.120
Технический комитет:: KŽP - Agricultural food products

Тип поставки:

Full version195 €Read online 24h10 €Read online 72h15 €

Язык документа:

ruen

Срок поставки:3 раб. дн. (перевод)

Цена:195 €

Описание (английский):

This International Standard specifies a method for the determination of the trypsin inhibitor activity (TIA) of soya products. This trypsin inhibitor activity is indicative of the degree of toasting of these products. The detection limit of the method is 0,5 mg/g.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

EN ISO 14902:2001 (ISO 14902:2001) specifies a laboratory method for the determination of trypsin inhibitor activity (TIA) in soya products used as animal feeding stuffs. TIA is used as an indicator of the degree of toasting/heat treatment of soy ingredients and affects protein digestibility in animal feeds. The method extracts inhibitors at pH 9.5, uses benzoyl-L-arginine-p-nitroanilide (L‑BAPA) as substrate, and measures released p‑nitroaniline spectrometrically (410 nm). The detection limit of the method is 0.5 mg/g.

Key Topics

Scope and purpose

Quantitative determination of TIA in soya products to assess processing quality and potential antinutritional effects.

Analytical principle

Extraction of trypsin inhibitors → inhibition of bovine trypsin → hydrolysis of L‑BAPA → spectrometric measurement of p‑nitroaniline.

Sample preparation and extraction

Grinding to minimize heat, extraction at alkaline pH (pH 9.5 ± 0.1), refrigerated extraction overnight (15–24 h).

Reagents and equipment

Use of analytical-grade reagents (Tris buffer, CaCl2, L‑BAPA, bovine trypsin), spectrometer at 410 nm, controlled water bath (≈37 °C), centrifuge (≈1 500 g), 10 mm cuvettes.

Quality controls

Check trypsin activity of each batch; use blanks and standards; aim for inhibition results in the 40–60% range via appropriate dilutions.

Performance characteristics

The standard includes guidance on repeatability, reproducibility and interlaboratory results.

Applications and Users

Who uses EN ISO 14902

Feed testing laboratories, soy meal and textured soy protein manufacturers, animal nutritionists, quality assurance teams in feed mills, regulatory authorities.

Practical uses

Verify processing adequacy (toasting) of soya products.
Assess antinutritional factor levels impacting protein digestibility in livestock diets.
Support product specification, batch release, supplier verification and compliance testing.

Related Standards

ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).

ISO 6497 - Recommended sampling methods for animal feeding stuffs (sampling guidance referenced but not part of the method).

By following EN ISO 14902:2001, laboratories can generate reproducible TIA data to support feed safety, nutritional formulation and process control for soya-based animal feed ingredients.

SIST EN ISO 14902:2002 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications and Users

Related Standards

Технические детали

ISO 14902:2001

Animal feeding stuffs — Determination of trypsin inhibitor activity of soya products

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

SIST EN ISO 6497:2005

Animal feeding stuffs - Sampling (ISO 6497:2002)