SIST EN ISO 16387:2024 PDF

Overview

EN ISO 16387:2023 - "Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) - Determination of effects on reproduction" - is an international/CEN standard for assessing the habitat function of soils using a chronic reproduction test with Enchytraeidae (Enchytraeus sp.). The method evaluates effects of soil contaminants and test substances taken up by dermal and alimentary routes under temperate conditions. It is intended for soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, post-remediation soils, agricultural sites, and wastes).

Key topics and technical requirements

• Test principle: chronic reproduction assessment of adult Enchytraeus sp. to detect sublethal and reproductive effects.
• Scope limits: not applicable to substances with an air/soil partition coefficient > 1 or vapour pressure > 300 Pa (at 25 °C). The method does not include monitoring of test substance persistence.
• Test design: includes range-finding and definitive tests, preparation of test mixtures (contaminated soils or substances added to substrate), controls, and use of reference substances.
• Measurements & endpoints: adult survival and reproduction (reproduction is the primary endpoint); calculation and expression of results (e.g., NOEC determination and statistical analysis).
• Technical elements covered: reagents, apparatus, test environment, addition of food and biological material, test conditions and measurements, validity criteria, and statistical assessment.
• Support material: annexes describe culture conditions, alternative Enchytraeus species, water holding capacity determination, extraction techniques (including a method for Enchytraeus crypticus), and statistical overview for NOEC.

Practical applications

• Soil ecotoxicity testing to complement chemical analyses when assessing habitat function and biological risk from contaminants.
• Contaminated site assessment and prioritization of remediation needs by detecting effects on soil fauna reproduction.
• Remediation verification and monitoring to compare pre- and post-treatment biological responses.
• Regulatory and product testing for wastes, soil amendments or substances that might affect soil biota.
• Laboratory method standardization for consistent, reproducible reproduction tests using Enchytraeus spp.

Who should use this standard

• Environmental laboratories and ecotoxicologists conducting soil toxicity assays.
• Regulators and risk assessors evaluating contaminated land and waste management.
• Remediation consultants, soil scientists, and environmental monitoring teams.
• Researchers studying soil invertebrate responses and population-level effects.

Related standards

• ISO 15799 (overview of test systems for soil ecotoxicology) and OECD terrestrial test guidelines referenced for methodological alignment and context.