SIST EN ISO 4833-2:2013/A1:2022 PDF

Name: SIST EN ISO 4833-2:2013/A1:2022 — Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 °C by the surface plating technique - Amendment 1: Clarification of scope (ISO 4833-2:2013/Amd 1:2022)
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Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 °C by the surface plating technique - Amendment 1: Clarification of scope (ISO 4833-2:2013/Amd 1:2022)

Название (английский):

SIST EN ISO 4833-2:2013/A1:2022

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 8
Дата публикации:: 7 марта 2022 г.
ICS:: 07.100.30
Технический комитет:: KŽP - Agricultural food products

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Срок поставки:2 раб. дн. (перевод)

Цена:120 €

Описание (английский):

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Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты SIST

Overview

EN ISO 4833-2:2013/A1:2022 (CEN endorsement of ISO 4833-2:2013/Amd 1:2022) is an amendment that clarifies the scope of the horizontal method for the enumeration of microorganisms by colony count at 30 °C using the surface plating technique. The standard specifies a horizontal, aerobic surface-plating method for microorganisms able to form colonies on solid media after incubation at 30 °C and confirms intended application across the food chain.

Key topics and technical requirements

Method type: Horizontal method - colony count by surface plating (manual spread plating) and includes the use of an automated spiral plater (see Annex A) for surface colony counts.

Incubation conditions: Aerobic incubation at 30 °C.

Organisms covered: Microorganisms able to grow and form colonies on the surface of solid medium under the specified conditions.

Minimum count guideline: Technique is suitable for samples that yield a minimum of 10 colonies per plate, corresponding broadly to expected levels of >100 cfu/ml for liquid samples or >1 000 cfu/g for solid samples.

Scope of application: Applies to products intended for human consumption, products for animal feeding (including pets), environmental samples from food and feed production/handling areas, and all samples from primary production.

Suitability notes and limitations: Especially suited to samples with heat‑sensitive organisms (e.g., psychrotrophs), obligately aerobic bacteria (e.g., Pseudomonas species), samples with small particles or intense colour that hamper pour-plate reading, and when colony-type differentiation is required. The amendment notes limited suitability for some fermented foods and certain animal feeds; alternative media or incubation conditions may be more appropriate for those products.

Applications and who uses it

Quality control and microbiology laboratories performing routine total viable counts for foods and feed.

Food safety and QA/QC teams in food manufacturing (fresh, chilled, frozen, dried foods).

Feed producers and animal-health labs monitoring feed hygiene.

Environmental monitoring programs in food and feed production and handling facilities.

Primary producers and regulators requiring standardized colony-count methods for compliance and surveillance.

Related standards and context

This amendment is part of the ISO 4833 series (Microbiology of the food chain). It was approved by CEN and is to be implemented as a national standard across CEN member states. The document emphasizes minimizing deviations from the horizontal method so results remain comparable across applications.

Keywords: EN ISO 4833-2:2013/A1:2022, colony count 30 °C, surface plating technique, horizontal method, enumeration of microorganisms, food microbiology, spiral plater, cfu/ml, cfu/g.

SIST EN ISO 4833-2:2013/A1:2022 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and who uses it

Related standards and context

Технические детали

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