SIST EN ISO 6579-1:2017 PDF

Overview

EN ISO 6579-1:2017 / ISO 6579-1:2017 - Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp. - is a standardized laboratory method published by CEN/ISO for the reliable detection of Salmonella in the food chain. The method applies to products intended for human consumption and animal feed, environmental samples from food production and handling areas, and primary production samples (for example animal faeces, dust and swabs). It is a horizontal protocol intended to detect most Salmonella serovars; some serovars may need additional culture steps.

Key topics and technical requirements

• Scope and targets: Detection of Salmonella spp. in foods, feeds, environmental and primary production samples.
• Workflow stages: pre-enrichment in non-selective medium → selective enrichment → selective plating → biochemical and serological confirmation.
• Selective enrichment options: choice between Rappaport-Vassiliadis broth (RVS) or modified semi-solid Rappaport-Vassiliadis agar (MSRV) for food, feed and environmental samples.
• MSRV limitation: MSRV is intended for detection of motile Salmonella and is not appropriate for non‑motile strains.
• Temperature: non-selective incubation range extended to 34 °C – 38 °C.
• Confirmation strategy: testing of suspect colonies with biochemical and serological methods; acceptable to confirm a single suspect colony initially - if negative, four additional suspect isolates from different media combinations must be tested.
• Special cases: detection procedures for Salmonella Typhi and Paratyphi are specified in Annex D.
• Cross-references: preparations of initial suspensions now reference ISO 6887; ISO 6785 has been incorporated into this edition.

Applications and users

• Food safety laboratories validating or routine-testing food and feed for Salmonella contamination.
• Quality assurance and regulatory testing in food production, processing and distribution.
• Environmental monitoring teams in food plants conducting surface and environmental sampling.
• Veterinary and primary production testing (faeces, farm swabs).
• Microbiology method developers and validation specialists working on Salmonella detection and serotyping.

Related standards

• Supersedes: ISO 6579:2002 (and related amendments).
• Incorporated: ISO 6785.
• Referenced for sample preparation: ISO 6887.
• Adopted by CEN as EN ISO 6579-1:2017.

Keywords: ISO 6579-1:2017, EN ISO 6579-1, Salmonella detection, food microbiology, MSRV, RVS, selective enrichment, food chain testing, Salmonella Typhi annex.