SIST ISO 10706:2010 PDF

Overview

ISO 10706:2000 specifies a laboratory method for determining the long‑term (chronic) sublethal toxicity of chemicals, industrial or sewage effluents and surface/ground waters to the freshwater crustacean Daphnia magna. Adapted from OECD Guideline 211, the standard describes a 21‑day exposure test that records parental survival and reproductive output (offspring per surviving parent) under controlled conditions to assess chronic water quality impacts.

Key topics and technical requirements

• Test organism & age: Daphnia magna females, less than 24 h old (2nd–5th brood), from healthy parthenogenetic cultures.
• Exposure duration: 21 days (semi‑static or flow‑through systems).
• Endpoints: parental survival and number of live offspring per live parent at test end; results compared to controls.
• Environmental conditions:
  • Temperature: 18–22 °C, variability ≤ 2 °C.
  • Photoperiod: 16 h light / 8 h dark; light intensity 600–800 lux (not > 1 200 lux).
  • Dissolved oxygen: > 3 mg/L; pH 6–9 with ≤ 1.5 pH units change during the test.
  • Hardness: ≥ 140 mg/L (as CaCO3) to support reproductive performance.
  • Exposure solutions shall not be aerated.
• Dilution water: synthetic media (OECD M4/M7) or ASTM reconstituted hard freshwater (Annexes A & B). TOC recommendations and notes on EDTA use for metal testing are provided.
• Sample handling: sampling per ISO 5667‑2 and ISO 5667‑16. Ideal testing within 12 h; cool 0–4 °C and test within 24 h if delayed; freeze (< −18 °C) if >48 h, with frozen samples usable up to 2 months. Example volume guidance given for semi‑static tests.
• Preparation of test solutions: guidance on stock solutions, dispersants/solvents (if used, solvent in highest exposure ≤ 0.1 ml/L), ultrasonic dispersion for poorly soluble substances, and limits on stock additions.
• Quality & analysis: controls, selection of concentration series, renewal schedule (if semi‑static), feeding, observations, validity criteria and data analysis (including time‑weighted mean concentration, Annex D).

Applications and users

ISO 10706 is used for:

• Chronic ecotoxicity testing of chemicals and effluents
• Regulatory compliance and environmental risk assessment
• Effluent monitoring by wastewater utilities
• Chemical registration and safety dossiers
• Research in ecotoxicology and environmental monitoring

Typical users: environmental testing laboratories, regulators, ecotoxicologists, chemical manufacturers, wastewater treatment operators and environmental consultancies.

Related standards

• ISO 5667‑2 (Sampling techniques)
• ISO 5667‑16 (Guidance on biotesting of samples)
• OECD Guideline 211 (source guideline)
• ASTM reconstituted hard freshwater (referenced in Annex B)

Keywords: ISO 10706, Daphnia magna, long term toxicity, chronic toxicity test, water quality, effluent testing, dilution water, OECD 211.