SIST ISO 14669:2010 PDF

Name: SIST ISO 14669:2010 — Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)
SKU: SIST ISO 14669:2010
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Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)

Название (английский):

SIST ISO 14669:2010

Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 19
Дата публикации:: 17 июня 2010 г.
ICS:: 13.060.70
Технический комитет:: KAV - Water quality

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Описание (английский):

This International Standard describes a method for the determination of the acute toxicity to one of three specified species of marine copepod (Copepoda, Crustacea) of a) chemical substances which are soluble, or can be maintained as a stable suspension or dispersion, under the conditions of the test; b) industrial or sewage effluents, treated or untreated, after decantation, filtration or centrifugation if necessary; c) marine or estuarine waters.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 14669:1999 specifies a standardized laboratory method for assessing acute lethal toxicity to marine copepods (Copepoda, Crustacea). The method determines median lethal concentrations (LC50) after 24 h and 48 h exposure to chemical substances (soluble or stably dispersed), industrial or sewage effluents, and marine/estuarine waters. The standard covers test design, culture and selection of test species, environmental conditions, sampling and sample preservation, and result reporting.

Key topics and technical requirements

Test organisms: one of three marine copepods - Acartia tonsa, Tisbe battagliai, or Nitocra spinipes. Specified life stages are required (e.g., A. tonsa stage 5 copepodids or adults; T. battagliai copepodids 6 ± 2 days; N. spinipes adults 3–4 weeks).

Endpoints: 24 h and 48 h LC50 (48 h LC50 is the primary endpoint); 96 h may be used where appropriate. Also record the lowest concentration causing 100% mortality and the highest with no mortality.

Test design: preliminary broad-range test (to define concentrations) followed by definitive test (replicated series). Minimum of 20 copepods per concentration in definitive tests (e.g., four replicates of five).

Environmental controls: temperature 20 °C ± 2 °C, 16 h/8 h light/dark photoperiod, appropriate seawater salinity and dissolved oxygen (>80% air saturation) and pH control. Copepod density limits are specified (not to exceed 1 per 0.5 ml; for A. tonsa recommended max 1 per 5 ml).

Sample and solution preparation: guidance on sampling (see ISO 5667-2, ISO 5667-16), stock solution preparation (including handling poorly soluble substances using ultrasonic or solvent assistance), and limit test at 100 mg/L or the maximum soluble concentration.

Quality control: use of a reference toxicant (recommended 3,5‑dichlorophenol) to verify culture sensitivity and method conformity. Record dissolved oxygen and pH at test start and end; scoring of mortality uses a 10 s observation rule for movement.

Applications and users

Environmental testing laboratories performing marine ecotoxicity and water quality testing

Regulatory agencies evaluating chemical hazards, effluent permits and environmental compliance

Industrial and municipal wastewater monitoring (effluent toxicity screening)

Environmental consultants and researchers conducting biotesting, impact assessments, and method development Practical uses include deriving LC50 values for risk assessment, screening effluents and chemicals for acute marine toxicity, and validating organism sensitivity with reference toxicants.

Related standards

ISO 5667-2 - Water quality - Sampling: guidance on sampling techniques

ISO 5667-16 - Water quality - Sampling: guidance on biotesting of samples

ISO 14669 includes informative annexes with example calculations, culture methods for the three copepod species, and precision data.

Keywords: ISO 14669, acute lethal toxicity, marine copepods, LC50, water quality testing, ecotoxicology, effluent testing, biotesting, marine toxicology.

SIST ISO 14669:2010 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards

Технические детали

ISO 14669:1999

Water quality — Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)

BS ISO 5667-21:2010

Water quality. Sampling - Guidance on sampling of drinking water distributed by tankers or means other than d…

SIST EN ISO 5667-16:2017

Water quality - Sampling - Part 16: Guidance on biotesting of samples (ISO 5667-16:2017)