SIST ISO 20481:2011 PDF

Name: SIST ISO 20481:2011 — Coffee and coffee products -- Determination of the caffeine content using high performance liquid chromatography (HPLC) -- Reference method
SKU: SIST ISO 20481:2011
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Coffee and coffee products -- Determination of the caffeine content using high performance liquid chromatography (HPLC) -- Reference method

Название (английский):

SIST ISO 20481:2011

Coffee and coffee products -- Determination of the caffeine content using high performance liquid chromatography (HPLC) -- Reference method

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 13
Дата публикации:: 16 мая 2011 г.
ICS:: 67.140.20
Технический комитет:: KŽP - Agricultural food products

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Описание (английский):

ISO 20481:2008 specifies a high performance liquid chromatography (HPLC) method for the determination of the caffeine content of: green coffee; roasted coffee; soluble coffee, regular and decaffeinated; and mixed instant coffee products (e. g. coffee/chicory mix or cappuccino-type coffee drink).

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 20481:2008 - "Coffee and coffee products - Determination of the caffeine content using high performance liquid chromatography (HPLC) - Reference method" defines a validated HPLC reference procedure for measuring caffeine in green coffee, roasted coffee, soluble (instant) coffee - regular and decaffeinated - and mixed instant coffee products (e.g., coffee/chicory blends, cappuccino-type powders). The method specifies sample preparation, extraction, chromatographic conditions and calculation rules to deliver traceable, repeatable caffeine results.

Key topics and technical requirements

Sample scope: Green beans, roasted beans, soluble coffee, decaffeinated and mixed instant products.

Extraction: Hot-water extraction at ≥90 °C with magnesium oxide (MgO) to protect column life; typically 5 g MgO and about 200 ml water per test portion, heated 20 minutes.

Chromatography: Reversed-phase C18 column (≥125 mm, ~5 µm, ≥5 000 theoretical plates), isocratic elution with a mobile phase of 24% (v/v) methanol in water.

Detection and injection: UV detection at ~272 nm (270–280 nm acceptable), typical flow rate 1.0 ml/min and injection volume 10 µl.

Standards and calibration: Stock caffeine standard 200 mg/l with working dilutions (≈40 mg/l for regular, ≈4 mg/l for decaf); 3–5 point calibration graph optional.

Quality and safety: Use HPLC-grade reagents and ISO 3696 grade 1 water; degas mobile phase; safety measures for methanol handling (gloves, eye protection, fume hood).

Results and reporting: Formulae for as‑is and dry‑basis caffeine (% mass) provided; report must reference ISO 20481 and include test details and any deviations.

Precision: Repeatability and reproducibility data from interlaboratory studies are supplied (Annex A / Table 1) to guide interpretation of r and R limits.

Applications and who uses it

Industrial QC laboratories at coffee roasters, instant coffee manufacturers and ingredient suppliers for product specification, batch release and supplier control.

Testing and certification bodies using a recognized reference method for regulatory compliance, labeling claims (caffeine content) and dispute resolution.

Research and R&D teams studying caffeine extraction, decaffeination processes or product formulation (mixes, beverages).

Customs/import labs and food safety authorities requiring harmonized methods for enforcement and trade.

Related standards

ISO 3696 - Water for analytical laboratory use

ISO 6673, ISO 11817, ISO 3726 - Moisture/dry matter methods for green, roasted and instant coffee (used when reporting dry‑basis results)

ISO 5725 series - Precision (repeatability, reproducibility) of measurement methods

Keywords: ISO 20481, caffeine determination, HPLC method, coffee testing, caffeine content, reference method, green coffee, roasted coffee, soluble coffee, decaffeinated, chromatographic analysis.

SIST ISO 20481:2011 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and who uses it

Related standards

Технические детали

ISO 20481:2008

Coffee and coffee products — Determination of the caffeine content using high performance liquid chromatograp…

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

SIST ISO 6673:2011

Green coffee -- Determination of loss in mass at 105 degrees C

SIST ISO 11817:1995

Roasted ground coffee -- Determination of moisture content -- Karl Fischer method (Reference method)

SIST ISO 3726:1995

Instant coffee -- Determination of loss in mass at 70 degrees C under reduced pressure

ISO 5725-6:1994/Cor 1:2001

Accuracy (trueness and precision) of measurement methods and results — Part 6: Use in practice of accuracy va…