SIST ISO 21427-1:2010 PDF

Name: SIST ISO 21427-1:2010 — Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei - Part 1: Evaluation of genotoxicity using amphibian larvae
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Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei - Part 1: Evaluation of genotoxicity using amphibian larvae

Название (английский):

SIST ISO 21427-1:2010

Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei - Part 1: Evaluation of genotoxicity using amphibian larvae

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 20
Дата публикации:: 2 июля 2010 г.
ICS:: 13.060.70
Технический комитет:: KAV - Water quality

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Описание (английский):

ISO 21427-1:2006 specifies a method for assessing genotoxicity using Amphibia larvae (Xenopus laevis and Pleurodeles waltl). The method is applicable to: aqueous effluents; aqueous leachates; eluates of soils; eluates of industrial waste; eluates of sludges from sewage treatment; surface and ground water; water-soluble substances.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 21427-1:2006 - "Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei - Part 1: Evaluation of genotoxicity using amphibian larvae" specifies a laboratory micronucleus assay using amphibian larvae (mainly Xenopus laevis, also Pleurodeles waltl) to assess genotoxic effects in aqueous samples. The method applies to aqueous effluents, leachates, eluates (soils, industrial waste, sludges), surface and ground water, and water‑soluble substances, providing a standardized protocol for environmental genotoxicity screening.

Key topics and technical requirements

Test organism and stage: Larvae of Xenopus laevis (recommended) or Pleurodeles waltl; tests start at Nieuwkoop and Faber stage 50 (larvae ~20–27 mm).

Exposure regime: Continuous exposure for 12 days to a range of sample concentrations, with paired negative (blank) and positive controls to validate biological responsiveness.

Endpoint: Frequency of erythrocytes with micronuclei, scored per 1 000 erythrocytes (expressed in ‰), using microscopic examination (immersion lens, high magnification).

Positive control: Cyclophosphamide monohydrate (reference substance); typical concentration referenced in the standard (analytical quality).

Test conditions: 12 h light / 12 h dark cycle; temperature controlled at 22 °C ± 1 °C; defined feeding, anaesthesia and staining procedures.

Sample handling: Sampling, storage and transport guidance (ISO 5667-16 referenced); interval between collection and lab receipt ≤ 24 h. For solids, EN 12457-2 leaching protocol is required (eluates must not be filtered), and eluates stored in the dark at 4 °C ± 3 °C and used within specified time limits.

Quality and validity criteria: Requirements for batch size, preconditioning of larvae, solvent controls (e.g., DMSO), and statistical processing of results (example methods provided in annexes).

Applications

Environmental monitoring of genotoxicity in surface and groundwater.

Assessment of industrial effluents, sewage sludges, landfill leachates, and contaminated soils (via eluates).

Regulatory compliance testing and risk assessment when screening for DNA‑damaging contaminants in aquatic environments.

Research and ecotoxicology studies comparing genotoxic potential of waterborne substances.

Related standards

ISO 21427-2 (Part 2: mixed population method using V79 cell line)

ISO 5667-16 (sampling - guidance on biotesting)

EN 12457-2 (leaching of solid wastes)

ISO 7346 series (acute fish toxicity methods)

Keywords: ISO 21427-1:2006, genotoxicity, micronuclei, amphibian larvae, Xenopus laevis, Pleurodeles waltl, water quality testing, effluent screening, leachate testing, aquatic genotoxicity.

SIST ISO 21427-1:2010 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications

Who should use this standard

Related standards

Технические детали

ISO 21427-1:2006

Water quality — Evaluation of genotoxicity by measurement of the induction of micronuclei — Part 1: Evaluatio…

SIST EN ISO 5667-16:2017

Water quality - Sampling - Part 16: Guidance on biotesting of samples (ISO 5667-16:2017)

BS EN ISO 21427-2:2009

Water quality. Evaluation of genotoxicity by measurement of the induction of micronuclei. Mixed population me…

SIST EN ISO 7346-2:1998

Water quality - Determination of the acute lethal toxicity of substances to a freshwater fish (Brachydanio re…