SIST ISO 6866:1995 PDF

Name: SIST ISO 6866:1995 — Animal feeding stuffs -- Determination of free and total gossypol
SKU: SIST ISO 6866:1995
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Animal feeding stuffs -- Determination of free and total gossypol

Название (английский):

SIST ISO 6866:1995

Animal feeding stuffs -- Determination of free and total gossypol

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 3
Дата публикации:: 1 мая 1995 г.
ICS:: 65.120
Технический комитет:: KŽP - Agricultural food products

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Описание (английский):

The method is applicable to cottonseed and cottonseed meals and cakes. The method consists in extracting of the compound in the presence of 3-aminopropane-1-ol, either with a mexture of 2-propanol and hexane for the determination of free gossypol or with dimethylformamide for the determination of total gossypol. Conversion to gossypol-dianiline using aniline, and measurement of the absorbance at the wavelength of maximum absorbance (between 435 and 445 nm).

Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты SIST

Overview

SIST ISO 6866:1995 specifies a standardized method for the determination of both free and total gossypol content in animal feeding stuffs, particularly those derived from cottonseed, such as cottonseed meals and cakes. Gossypol, a naturally occurring polyphenolic compound found in cotton plants, can have toxic effects on animals if present in high concentrations. The accurate measurement of gossypol levels is essential for ensuring the safety and quality of animal feeds. This standard supports feed manufacturers, laboratories, and regulatory agencies in maintaining animal health and complying with food safety regulations.

Key Topics

Scope of Application:

Applicable to cottonseed, cottonseed meal, cottonseed cakes, and compound animal feeds containing these ingredients.

Analytical Approach:

Extraction of gossypol in the presence of 3-aminopropane-1-ol.
Different extraction solvents for free (2-propanol/hexane mixture) and total (dimethylformamide) gossypol.
Conversion of extracted gossypol to gossypol-dianiline through aniline reaction.
Spectrophotometric measurement at maximum absorbance wavelength (435-445 nm) for quantification.

Detection Limit:

Free gossypol: 20 mg/kg
Total gossypol: 50 mg/kg

Sample Preparation:

Detailed procedures for preparing and handling test samples and reagents.
Requirements for blank and reference solutions to ensure measurement accuracy.

Result Calculation and Reporting:

Standardized formula for calculating gossypol content.
Criteria for repeatability and consistency between tests.
Comprehensive reporting requirements for laboratory testing.

Applications

Feed Safety Control:

Ensures that animal feeds derived from cottonseed products do not contain unsafe levels of gossypol, helping protect livestock against toxicity.

Regulatory Compliance:

Assists animal feed producers, suppliers, and control agencies in meeting national and international food safety standards.
Used for mandatory or voluntary certification, auditing, and quality assurance processes.

Analytical Laboratories:

Provides a reliable, repeatable method for laboratories testing raw materials and finished animal feed products.

Research and Development:

Supports R&D in animal nutrition and toxicology by providing a reference method for assessing the effects of gossypol in feed trials.

Related Standards

ISO 6497: Animal feeding stuffs - Sampling

Describes the procedures for sampling animal feeds to ensure that tested samples are representative and reliable.

ISO 6498: Animal feeding stuffs - Preparation of test samples

Covers the preparatory steps required to ensure feed samples are ready for chemical analysis.

Other Standards:

Additional ISO standards related to animal feed safety, chemical analysis, and quality control may complement the use of SIST ISO 6866:1995.

Practical Value

SIST ISO 6866:1995 serves as a critical tool for the animal feed industry in the reliable determination of gossypol levels, directly impacting animal health and food safety. By utilizing this standard, stakeholders can ensure product quality, meet regulatory demands, and maintain their reputation in international markets. Accurate gossypol analysis supports both commercial and governmental efforts to monitor and control potentially harmful contaminants in the animal feed supply chain.

SIST ISO 6866:1995 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Related Standards

Practical Value

Технические детали

SIST EN ISO 6497:2005

Animal feeding stuffs - Sampling (ISO 6497:2002)

SIST EN ISO 6498:2012

Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012)

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