Overview
SIST-TS CEN/TS 18267:2026 establishes comprehensive guidelines for the preparation, detection, identification, and characterization of nano-objects in inorganic food additives incorporated in food matrices. Developed by SIST under the structure of CEN, this technical specification addresses the needs of the food industry, service providers, and control laboratories to reliably analyze nano-objects in food products. The document focuses on two key analytical techniques-electron microscopy coupled with energy dispersive X-ray spectroscopy (EM-EDX) and single-particle inductively coupled plasma mass spectrometry (spICP-MS)-each selected for their ability to deliver detailed information on the chemical composition and number-based particle size distribution of inorganic nanoparticles.
Key Topics
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Sample preparation methodology:
Detailed procedures are provided for matrix digestion, extraction, and dilution, tailored to the specific inorganic additive, type of food matrix, and analytical method (EM-EDX or spICP-MS). Emphasis is placed on avoiding alteration of nanoparticle properties during these pre-analytical steps.
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Detection and identification of nano-objects:
Guidelines assist laboratories in effectively using EM-EDX and spICP-MS to detect and identify nano-objects within complex food matrices, considering challenges such as distinguishing target particles from background.
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Characterization and measurement:
The specification covers best practices for the characterization of nanoparticles, including assessment of chemical composition via EM-EDX and number-based size distribution through both microscopy and single-particle mass spectrometry.
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Applicability and limitations of analytical methods:
The document outlines the strengths and constraints of EM-EDX and spICP-MS, detailing scenarios where each technique is preferred.
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Protocol examples:
Informative annexes illustrate extraction and measurement protocols, such as for titanium dioxide (E 171) and iron oxides (E 172) in sugar shell confectionary and other matrices.
Applications
This technical standard supports practical implementation across multiple stakeholders in the food sector:
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Food industry quality control:
Ensures that nano-objects in inorganic additives can be reliably detected and quantified before market release, aiding compliance with European and international food safety regulations.
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Control laboratories:
Provides clear, reproducible methodologies for official testing of food samples, helping authorities confirm the presence and profile of nano-objects as required by regulations such as EU Regulation (EU) 2015/2283 on novel foods.
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Service providers and contract testing facilities:
Offers standardized approaches for third-party laboratories to support manufacturers in risk assessment, product development, and regulatory submissions related to nanoparticles in food.
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Research and development:
Facilitates scientific studies and innovation aimed at improving analytical techniques for nanotechnology applications in food matrices.
Related Standards
Organizations implementing SIST-TS CEN/TS 18267:2026 should also reference the following key standards:
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CEN/TS 17273:2018
Nanotechnologies – Guidance on detection and identification of nano-objects in complex matrices.
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CEN ISO/TS 19590:2024
Characterization of nano-objects using single particle inductively coupled plasma mass spectrometry.
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EN ISO 19749:2023
Measurements of particle size and shape distributions by scanning electron microscopy.
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EN ISO 21363:2022
Measurements of particle size and shape distributions by transmission electron microscopy.
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CEN ISO/TS 23302:2022
Requirements and recommendations for the identification of measurands characterizing nano-objects and nano-object-containing materials.
Referencing these standards alongside SIST-TS CEN/TS 18267:2026 will help ensure robust, harmonized methodologies for the analysis of nano-objects in food additives.
Keywords: nanotechnologies, food additives, nano-objects, EM-EDX, spICP-MS, sample preparation, particle size distribution, food safety, European standards, inorganic additives.