SIST EN ISO 18744:2016/A1:2026

Microbiology of the food chain - Detection and enumeration of Cryptosporidium and Giardia in fresh leafy green vegetables and berry fruits - Amendment 1: Method validation studies and performance characteristics (ISO 18744:2016/Amd 1:2026)

Название (английский):

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 14
Дата публикации:: 21 июля 2026 г.
Издание:: ISO 18744:2016/Amd 1:2026
ICS:: 07.100.30

Тип поставки:

Full version25 200 ₽Read online 24h1 200 ₽Read online 72h1 800 ₽

Язык документа:

Срок поставки:3 раб. дн. (перевод)

Цена:25 200 ₽

Описание (английский):

No scope available

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

SIST EN ISO 18744:2016/A1:2026 addresses the microbiological analysis of the food chain, focusing on the detection of Cryptosporidium and Giardia in fresh leafy green vegetables and berry fruits. This amendment refines the standard's methodology, emphasizing method validation studies and performance characteristics for qualitative detection. The standard supports improved food safety by guiding laboratories in accurately identifying these protozoan parasites, which are significant concerns for public health.

Key Topics

Detection of Cryptosporidium and Giardia The standard outlines procedures for identifying the presence of Cryptosporidium oocysts and Giardia cysts in selected fresh produce. Detection is qualitative, indicating presence or absence rather than enumeration.

Method Validation and Performance Amendment 1 introduces validated performance metrics-including specificity, sensitivity, and limit of detection (LOD)-based on interlaboratory studies. It highlights the reproducibility and reliability required for food microbiology testing across different laboratories.

Sample Types and Approach

Leafy green vegetables (e.g., lettuce)
Berry fruits (e.g., raspberries) Laboratories must use defined protocols for sample preparation, handling, and centrifugation procedures to ensure optimal recovery of target organisms.

Performance Reporting Results are to be reported as the presence or absence of the parasites per sample weight, along with supporting identification details as applicable.

Interlaboratory Study Insights

High reproducibility was observed for Cryptosporidium detection in lettuce and raspberries.
Variable reproducibility was observed for Giardia detection in lettuce, highlighting the need for careful laboratory validation.

Applications

Food Safety Laboratories Accredited laboratories use this standard for surveillance and outbreak investigations, supporting regulatory compliance and the verification of public health controls.

Quality Assurance in Food Production Producers and processors of fresh produce benefit from validated detection methods to minimize contamination risks, thus protecting brand integrity and consumer health.

Regulatory Compliance National authorities reference this standard for setting and verifying compliance with food safety regulations involving protozoan parasites.

Research and Epidemiological Studies Researchers use these protocols to assess contamination sources and prevalence, informing risk assessments and mitigation strategies in the food supply.

Related Standards

For comprehensive food microbiology and method validation, the following standards may also be relevant:

ISO 16140-2: Microbiology of the food chain - Method validation

ISO 6887: Preparation of test samples, initial suspension, and decimal dilutions for microbiological examination

ISO 7218: General requirements and guidance for microbiological examinations

ISO 21527: Enumeration of yeasts and molds in food

Practical Value

Assurance of result comparability through standardized validation criteria

Support for risk-based controls on fresh produce, where protozoan contamination has been of concern

Foundation for harmonized international trade in produce by meeting recognized detection protocols

By adhering to SIST EN ISO 18744:2016/A1:2026, laboratories and food industry stakeholders enhance their capacity to detect protozoan parasites in fresh foods, thereby ensuring higher food safety standards and consumer protection on a national and international scale.

SIST EN ISO 18744:2016/A1:2026

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Related Standards

Practical Value

Технические детали

ISO 18744:2016

Microbiology of the food chain — Detection and enumeration of Cryptosporidium and Giardia in fresh leafy gree…

BS EN ISO 16140-2:2016+A1:2024

Microbiology of the food chain. Method validation. Protocol for the validation of alternative (proprietary) m…

ISO 6887-3:2017/Amd 1:2020

Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for mi…

SIST EN ISO 7218:2024

Microbiology of the food chain - General requirements and guidance for microbiological examinations (ISO 7218…

ISO 21527-2:2008

Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of yeasts and moulds —…