EN ISO 17180:2013 PDF

Name: EN ISO 17180:2013 — Animal feeding stuffs - Determination of lysine, methionine and threonine in commercial amino acid products and premixtures (ISO 17180:2013)
SKU: EN ISO 17180:2013
Price: 65.56 EUR
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Animal feeding stuffs - Determination of lysine, methionine and threonine in commercial amino acid products and premixtures (ISO 17180:2013)

Название (английский):

EN ISO 17180:2013

Animal feeding stuffs - Determination of lysine, methionine and threonine in commercial amino acid products and premixtures (ISO 17180:2013)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 18
Дата публикации:: 17 апреля 2013 г.
Издание:: CEN EN 17180 edition 1 version 1
ICS:: 65.120

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Описание (английский):

ISO 17180:2013 specifies a method for the quantitative determination of free (non-protein-bound) lysine, methionine, and threonine in commercial products and premixtures containing more than about 10 % mass fraction of the respective amino acid. It does not distinguish between d- and l-forms.

Abstract

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Упомянутые в описании и другие стандарты EN

Overview

EN ISO 17180:2013 - "Animal feeding stuffs - Determination of lysine, methionine and threonine in commercial amino acid products and premixtures" - specifies a laboratory method for the quantitative determination of free (non‑protein‑bound) lysine, methionine and threonine in commercial amino‑acid products and premixtures. The method applies to materials containing roughly more than 10% mass fraction of the respective amino acid and does not distinguish between D‑ and L‑forms. The standard was prepared by ISO/TC 34 (Food products) with CEN/TC 327 and is published as EN ISO 17180:2013.

Key topics and technical requirements

Scope: Quantitative assay of free lysine, methionine and threonine in trade amino‑acid products and feed premixtures (≈ >10%).

Analytical principle:

Extraction in dilute hydrochloric acid, dilution with sodium citrate buffer.
Internal standard: norleucine.
Separation by amino acid analyser or HPLC using a cation‑exchange column and sodium citrate‑based eluents.
Post‑column derivatization with ninhydrin (colorimetric detection) or OPA (fluorescence detection).

Reagents & materials: High‑purity amino acid and norleucine standards, sodium citrate buffer (pH ~2.20), 0.1 mol/L HCl for extraction, appropriate elution buffers and post‑column reagents.

Apparatus: Cation exchange column (oven), guard column, injection system (10–100 µL), pumps for buffer delivery and post‑column reagent, detectors (UV at 440/570 nm for ninhydrin; fluorescence excitation 330 nm/emission 460 nm for OPA), membrane filters and analytical balance.

Procedure essentials: Sample grinding (≤0.25 mm), prepare two independent extracts per sample, perform either weight or volumetric dilutions for calibration, and calculate results using internal standard normalization.

Precision & reporting: The standard includes precision data from collaborative trials and requirements for test reporting.

Applications and who uses it

Feed manufacturers: quality control of commercial amino‑acid products and premixtures before release.

Analytical & testing laboratories: routine quantification of free lysine, methionine and threonine in raw materials and finished premixtures.

Regulatory bodies and auditors: verifying label claims and compliance with feed composition requirements.

Amino‑acid suppliers and formulators: confirming purity, concentration and batch consistency.

Practical uses include batch release testing, supplier qualification, formulation verification and troubleshooting of feed nutrient supply.

Related standards

EN ISO 17180:2013 is the CEN‑endorsed version of ISO 17180:2013. It is intended to be used alongside general sampling and feed analysis standards (ISO/CEN methods for sampling and other compositional analyses) and the manufacturer’s instrument‑specific HPLC/AA analyser method guidance.

Keywords: EN ISO 17180:2013, lysine analysis, methionine threonine, amino acid analysis, animal feeding stuffs, HPLC, amino acid analyser, premixtures, feed quality control.

EN ISO 17180:2013 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and who uses it

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