ISO 15174:2012 PDF

Name: ISO 15174:2012 — Milk and milk products — Microbial coagulants — Determination of total milk-clotting activity
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Milk and milk products — Microbial coagulants — Determination of total milk-clotting activity

Название (английский):

ISO 15174:2012

Milk and milk products — Microbial coagulants — Determination of total milk-clotting activity

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 9
Дата публикации:: 3 мая 2012 г.
Издание:: ISO IS 15174 edition 2 version 1
ICS:: 07.100.30

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Описание (английский):

ISO 15174:2012 specifies a method for comparison of the total milk-clotting activity of a microbial coagulant sample with the milk-clotting activity of an international microbial coagulant reference standard on a standard milk substrate prepared with a calcium chloride solution of concentration 0,5 g/l (pH ~6,5).

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 15174:2012 - Milk and milk products - Microbial coagulants - Determination of total milk‑clotting activity defines an international, comparative method to measure the total milk‑clotting activity of microbial coagulants. The method compares a test sample against an international microbial coagulant reference standard (Rhizomucor miehei) using a defined standard milk substrate prepared with calcium chloride (0.5 g/l, pH ≈ 6.5). The principle is based on measuring the time to visible flocculation (clotting) under controlled chemical and physical conditions.

Key topics and technical requirements

Scope and principle: Relative determination of milk‑clotting activity by comparing clotting times of test and reference solutions.

Reference standard: R. miehei powder ampoules with labeled total milk‑clotting activity (set relative to an earlier rennet standard). Stored at −18 °C, protected from moisture and light.

Substrate preparation: Standard milk substrate prepared with calcium chloride working solution (0.5 g/l; pH ~6.5).

Critical reagents: Buffer pH 5.5 (sodium acetate/acetic acid), calcium chloride stock (500 g/l) and working solutions, low‑heat low‑fat spray‑dried milk powder.

Apparatus and tolerances:

Micropipette (0.5 ml delivery, high repeatability)
One‑mark volumetric flasks and pipettes (class A)
Calibrated thermometer (20–45 °C, ±0.1 °C), pH meter (0.01 pH), analytical balance (1 mg), stopwatch (1 s)
Water bath at 32 °C ±1 °C with rotating spindle and lamp for visual observation (rotation ~2–4 r/min suggested)

Sample handling: Procedures for liquid and powdered coagulants (mixing, temperature equilibration to 18–22 °C); sampling guidance referenced to ISO 707.

Results and quality: Calculation and expression of activity relative to the reference; documented precision descriptors (interlaboratory, repeatability, reproducibility) and required test reporting.

Applications and users

ISO 15174:2012 is used where reproducible measurement of microbial milk‑clotting activity is needed:

Dairy laboratories performing quality control and batch certification of microbial coagulants

Cheese manufacturers for dose standardization and process control

Enzyme producers and suppliers to label and certify product activity (comparability across batches)

R&D groups evaluating enzyme performance, sensitivity to pH, calcium and temperature

Regulatory and standards bodies concerned with analytical consistency in the dairy sector

Practical benefits include consistent dosing in cheese‑making, reliable supplier comparisons, and standardized enzyme potency evaluation.

Related standards

ISO 11815 / IDF 157 - relative milk‑clotting activity test for bovine rennets (method alignment)

ISO 15163 / IDF 110 - qualitative determination of microbial coagulants (annex referenced)

ISO 707 / IDF 50 - sampling of liquid and powdered coagulants

Keywords: ISO 15174:2012, microbial coagulants, milk‑clotting activity, R. miehei, calcium chloride 0.5 g/l, standard milk substrate, cheese‑making, enzyme activity testing.

ISO 15174:2012 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards

Технические детали

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)

ISO 11815:2007

Milk — Determination of total milk-clotting activity of bovine rennets

ISO 15163:2012

Milk and milk products — Calf rennet and adult bovine rennet — Determination by chromatography of chymosin an…