ISO 17375:2006 PDF

Name: ISO 17375:2006 — Animal feeding stuffs — Determination of aflatoxin B1
SKU: ISO 17375:2006
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Animal feeding stuffs — Determination of aflatoxin B1

Название (английский):

ISO 17375:2006

Animal feeding stuffs — Determination of aflatoxin B1

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 11
Дата публикации:: 9 июня 2006 г.
Издание:: ISO IS 17375 edition 1 version 1
ICS:: 65.120

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Описание (английский):

ISO 17375:2006 specifies a method for the determination of aflatoxin B1 in animal feeding stuffs using high-performance liquid chromatography with post-column derivatization. It is applicable to animal feeding stuffs with a fat content of up to 50 %. The limit of quantification of this method has been demonstrated to be better than 0,5 g/kg for aflatoxin B1 for a signal-to-noise ratio of 6.

Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты ISO

Overview - ISO 17375:2006 and aflatoxin B1 testing in feed

ISO 17375:2006 specifies a validated laboratory method for the determination of aflatoxin B1 in animal feeding stuffs. The method uses reverse-phase high-performance liquid chromatography (RP‑HPLC) with post‑column derivatization (PCD) and fluorescence detection. It is applicable to feed matrices with a fat content up to 50%, and the demonstrated limit of quantification (LOQ) is better than 0.5 µg/kg for a signal‑to‑noise ratio of 6.

Key topics and technical requirements

Analytical principle: extraction with acetone/water, immunoaffinity column (IAC) cleanup, RP‑HPLC separation, PCD via bromination, fluorescence detection.

Immunoaffinity columns (IACs): antibodies specific to aflatoxin B1; minimum capacity ≥ 40 ng; recovery ≥ 80% for 0.25 ng spike (in acetone/water).

Post‑column derivatization options:

Electrochemically generated bromine, or
Chemical reagent pyridinium hydrobromide perbromide (PBPB).

Mobile phases: defined water/acetonitrile/methanol mixtures; one option (for electrochemical PCD) contains potassium bromide and nitric acid.

Detection: fluorescence detector (recommended Ex ≈ 365 nm, Em ≈ 435 nm).

Calibration and quantification: prepared aflatoxin B1 stock and working solutions, procedures for calibration curves and two calibration options (A and B).

Performance and quality: method precision established by interlaboratory testing; repeatability and reproducibility parameters defined in the standard.

Safety and good laboratory practice: use of toxic, flammable solvents (acetone, methanol, acetonitrile); aflatoxins are carcinogenic and light‑sensitive; acid‑washed glassware recommended to prevent analyte loss.

Practical applications and who uses ISO 17375

Feed testing laboratories performing routine surveillance and compliance testing for aflatoxin B1 in animal feed.

Regulatory authorities and food safety agencies that require standardized, validated methods for enforcement and monitoring.

Feed manufacturers and quality control teams ensuring raw materials and finished feeds meet safety specifications.

Contract analytical labs and research groups studying mycotoxin contamination, mitigation, and risk assessment. Benefits include a reproducible HPLC‑based protocol, clear cleanup (IAC) and derivatization options, and an LOQ suitable for low‑level monitoring of aflatoxin B1.

Related standards and references

ISO 3696:1987 (laboratory water quality) is referenced for water used in reagents and mobile phases.

Prepared by ISO/TC 34 (Food products), Subcommittee SC 10 (Animal feeding stuffs).

Keywords: ISO 17375, aflatoxin B1, animal feeding stuffs, HPLC, post‑column derivatization, immunoaffinity column, PBPB, electrochemical bromination, fluorescence detection, feed testing.

ISO 17375:2006 PDF

Abstract

Похожие стандарты

Overview - ISO 17375:2006 and aflatoxin B1 testing in feed

Key topics and technical requirements

Practical applications and who uses ISO 17375

Related standards and references

Технические детали

ISO 3696:1987

Water for analytical laboratory use — Specification and test methods

SIST EN ISO 17375:2006

Animal feeding stuffs - Determination of aflatoxin B1 (ISO 17375:2006)

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