ISO 18252:2006 PDF

Name: ISO 18252:2006 — Anhydrous milk fat — Determination of sterol composition by gas liquid chromatography (Routine method)
SKU: ISO 18252:2006
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Anhydrous milk fat — Determination of sterol composition by gas liquid chromatography (Routine method)

Название (английский):

ISO 18252:2006

Anhydrous milk fat — Determination of sterol composition by gas liquid chromatography (Routine method)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 13
Дата публикации:: 3 февраля 2006 г.
Издание:: ISO IS 18252 edition 1 version 1
ICS:: 67.100.01

Тип поставки:

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Язык документа:

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Срок поставки:3 раб. дн. (перевод)

Цена:195 €

Описание (английский):

ISO 18252|IDF 200:2006 specifies a routine gas liquid chromatographic method for the determination of the sterol composition in anhydrous milk fat extracted from dairy products directly on the unsaponifiable matter, without purification and derivatization.

Abstract

Overview

Key topics and technical requirements

Scope: Routine determination of sterol composition in anhydrous milk fat; validated for samples containing ~28–32% vegetable fat.
Principle: Addition of 5α‑cholestane as internal standard → saponification with methanolic KOH (2 mol/L) → extraction of the unsaponifiable matter with diethyl ether → analysis by capillary gas chromatography (GLC/GC) with flame ionization detection (FID).
Standards/reagents: Reference sterols (cholesterol, campesterol, stigmasterol, β‑sitosterol) and prepared standard solutions; use of analytical-grade solvents (n‑hexane, ethanol, methanol) and anhydrous Na2SO4.
Instrumentation: Fused silica capillary columns, split or on‑column injectors, temperature-programmable oven (up to ~310 °C in examples), carrier gases (He, H2 or N2) and computerized integration.
Performance and limitations: The method provides repeatability and reproducibility data (interlaboratory precision reported in the standard). Because there is no purification step, the method requires caution for milk‑fat purity verification; suspicious samples should be analyzed using the reference method ISO 12078/IDF 159.
Safety: Handling flammable solvents and heated GC equipment - comply with relevant laboratory safety rules.

Applications and users

Who uses it: Food chemistry laboratories, dairy quality control teams, regulatory laboratories, and lipid analysts involved in milk and dairy product testing.
Practical applications:
- Quantifying cholesterol in anhydrous milk fat for nutritional labeling and research.
- Detecting and profiling phytosterols to assess vegetable‑fat adulteration in milk fat blends.
- Routine quality control of butter, milk powders, ghee and other anhydrous-dairy-fat products.
- Supporting authenticity and compositional surveillance in dairy supply chains.

Related standards

ISO 12078 / IDF 159 - reference method for sterol analysis (recommended if purity is in question).
ISO 14156 / IDF 172 - extraction methods for lipids and liposoluble compounds.
ISO 3696 - water for analytical laboratory use.
ISO 707 / IDF 50 - recommended sampling for milk products.

Keywords: ISO 18252, anhydrous milk fat, sterol composition, gas liquid chromatography, GLC, GC, cholesterol, phytosterols, IDF 200, dairy analysis, unsaponifiable matter.

Технические детали

Технический комитет: ISO/TC 34/SC 5 - Milk and milk products
SKU: ISO 18252:2006

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ISO 12078:2006

Действующий

Anhydrous milk fat — Determination of sterol composition by gas liquid chromatography (Reference method)

Overview ISO 12078:2006 (IDF 159:2006) is a reference method for determining the sterol composition of anhydrous milk fat using gas‑liquid chromatography (GLC). The standard defines a validated labor…

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ISO 14156:2001/Amd 1:2007

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Milk and milk products — Extraction methods for lipids and liposoluble compounds — Amendment 1

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ISO 18252:2006 PDF

Abstract

Overview

Похожие стандарты

Key topics and technical requirements

Applications and users

Related standards

Технические детали

ISO 12078:2006

Anhydrous milk fat — Determination of sterol composition by gas liquid chromatography (Reference method)

ISO 14156:2001/Amd 1:2007

Milk and milk products — Extraction methods for lipids and liposoluble compounds — Amendment 1

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)

SIST EN ISO 1825:2026

Rubber hoses and hose assemblies for aircraft ground fuelling and defuelling - Specification (ISO 1825:2026)