ISO 19563:2017 PDF

Name: ISO 19563:2017 — Determination of theanine in tea and instant tea in solid form using high-performance liquid chromatography
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Determination of theanine in tea and instant tea in solid form using high-performance liquid chromatography

Название (английский):

ISO 19563:2017

Determination of theanine in tea and instant tea in solid form using high-performance liquid chromatography

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 13
Дата публикации:: 10 января 2017 г.
Издание:: ISO IS 19563 edition 1 version 1
ICS:: 67.140.10

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Описание (английский):

ISO 19563:2016 specifies a high-performance liquid chromatographic (HPLC) method for the determination of theanine content in tea (Camellia sinensis). It is applicable to both tea and instant tea samples. Separation of L- and D-theanine is not possible using this method; however, the L-enantiomer is the major form in tea.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview - ISO 19563:2017 (Theanine analysis by HPLC)

ISO 19563:2017 specifies a validated high-performance liquid chromatography (HPLC) method for the quantitative determination of theanine in tea (Camellia sinensis) and instant tea in solid form. The procedure covers sample preparation, optional polyamide clean-up to remove polyphenols, chromatographic conditions using a reversed-phase C18 column, and method performance characteristics (precision, repeatability, reproducibility). The method does not separate L- and D‑theanine; L‑theanine is the predominant enantiomer in tea.

Key technical topics and requirements

Scope: Applicable to leaf tea and instant tea samples; results expressed per dry matter.

Principle: Reversed-phase (RP) C18 column with UV detection at 210 nm; water and acetonitrile used as mobile phases.

Reagents & standards: Grade 1 water (ISO 3696), HPLC‑grade acetonitrile, methanol, polyamide (Polyamide CC 6) for clean-up, and anhydrous L‑theanine standard (>99%).

Calibration range: Working standards prepared in the 5 µg/ml to 200 µg/ml range (from a 1 mg/ml stock).

Sample preparation:

Leaf tea: 1.00 g brewed in 100 ml boiling water for 5 min, cooled, made to volume and filtered (0.45 µm) or centrifuged.
Instant tea: 0.100 g dissolved in ≈50 °C water, sonicated, filtered or centrifuged.

Optional clean-up: Polyamide column chromatography to retain polyphenols while eluting theanine - recommended where interfering peaks occur.

Chromatography: Binary gradient-capable HPLC with thermostatted column compartment; suggested column (e.g., Phenomenex Aqua 250 × 4.6 mm or 2 mm equivalent). Flow rates: ~1.0 ml/min for 4.6 mm columns, ~0.25 ml/min for 2 mm columns.

Method performance: Includes interlaboratory test data and stated repeatability/reproducibility criteria.

Practical applications and users

Quality control in tea production and instant tea manufacturing - routine monitoring of theanine content to support flavor and product specifications.

Food testing laboratories and regulatory bodies conducting compositional analysis or compliance testing.

Research labs studying tea chemistry, sensory compounds (umami), or nutritional content of tea-derived products and supplements.

Dietary supplement and novel food developers assessing theanine levels in ingredient sourcing and product formulation.

Related standards and references

ISO 1839 (Tea - Sampling), ISO 1572 / ISO 1573 (sample preparation & moisture/dry matter), ISO 7513 / ISO 7516 (instant tea sampling and moisture). These referenced standards support representative sampling and dry-matter correction required by ISO 19563:2017.

Keywords: ISO 19563:2017, theanine analysis, HPLC, tea analysis, Camellia sinensis, L-theanine, polyamide clean-up, reversed-phase C18, UV 210 nm, instant tea.

ISO 19563:2017 PDF

Abstract

Похожие стандарты

Overview - ISO 19563:2017 (Theanine analysis by HPLC)

Key technical topics and requirements

Practical applications and users

Related standards and references

Технические детали

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

BS EN ISO 18395:2007

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BS ISO 157:2025

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BS ISO 15734:2001

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ISO 7513:1990/Amd 1:2012

Instant tea in solid form — Determination of moisture content (loss in mass at 103 degrees C) — Amendment 1

ISO 7516:1984

Instant tea in solid form — Sampling