ISO 19827:2016 PDF

Name: ISO 19827:2016 — Water quality — Determination of the acute toxicity to the freshwater rotifer Brachionus calyciflorus
SKU: ISO 19827:2016
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Water quality — Determination of the acute toxicity to the freshwater rotifer Brachionus calyciflorus

Название (английский):

ISO 19827:2016

Water quality — Determination of the acute toxicity to the freshwater rotifer Brachionus calyciflorus

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 13
Дата публикации:: 18 февраля 2016 г.
Издание:: ISO IS 19827 edition 1 version 1
ICS:: 13.060.70

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Описание (английский):

ISO 19827:2016(E) specifies a method for the determination of the lethal effects of toxicants to Brachionus calyciflorus after 24 h exposure. The method is applicable to: a) chemical substances which are soluble, or which can be maintained as a stable suspension of dispersions under the conditions of the test; b) industrial or sewage effluents, treated or untreated, if appropriate after decantation, filtration or centrifugation; c) freshwaters; d) aqueous extracts.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 19827:2016 - "Water quality - Determination of the acute toxicity to the freshwater rotifer Brachionus calyciflorus" - specifies a standardized 24-hour bioassay to determine lethal effects (24 h LC50) of toxicants on the freshwater rotifer Brachionus calyciflorus. The method covers soluble chemicals, stable suspensions, industrial or sewage effluents (after appropriate treatment), freshwaters and aqueous extracts. Tests are performed under controlled laboratory conditions and are intended for reliable aquatic toxicity data for water quality assessment and ecotoxicology.

Key Topics and Technical Requirements

Test organism: Female Brachionus calyciflorus from lab cultures or hatched from cysts (hatching procedure in Annex A).

Exposure: 24-hour acute exposure with mortality assessment and calculation of 24 h LC50.

Test design:

Minimum five concentrations plus control; geometric dilution series.
Range-finding: typical separation factor 10 for chemicals; effluents commonly use 1:1 dilutions.
Definitive test: dilution factor not exceeding 3.2 for chemicals; effluents/waters typically 1:1.
Replication: six replicates per concentration and control (microplate 48-well format recommended).

Environment & apparatus:

Incubation in the dark at 25 ± 1 °C.
Use of disposable 48-well microplates, stereomicroscope, pipettes and temperature-controlled chamber.

Samples & preparation:

Sampling and handling guidance (ISO 5667-16 referenced): test ideally within 12 hours; cooling/freezing protocols given if delays occur.
Decantation, filtration or centrifugation where necessary to remove interfering particulates.

Controls & references:

Reference substances: potassium dichromate (K2Cr2O7) or copper sulfate (CuSO4·5H2O). Note safety caution for K2Cr2O7.

Measurements & reporting:

Dissolved oxygen must be measured (ISO 5814 referenced); data analysis guidance (ISO/TS 20281 referenced).
Annexes include artificial water recipe (Annex B) and performance data (Annex C).

Applications

Regulatory compliance testing for effluents and surface waters.

Ecotoxicological evaluation of chemicals, formulations and wastewater discharges.

Environmental monitoring, risk assessment and product safety screening using a sensitive zooplankton bioassay.

Comparative toxicity screening in laboratory and contract testing environments.

Related Standards

ISO 5667-16 - Sampling guidance for biotesting

ISO 5814 - Dissolved oxygen measurement

ISO/TS 20281 - Statistical interpretation of ecotoxicity data

ISO 19827 provides a reproducible, recognized rotifer acute toxicity test (24 h LC50) for water quality and ecotoxicology applications, helping stakeholders generate comparable, regulatory-grade aquatic toxicity data.

ISO 19827:2016 PDF

Abstract

Похожие стандарты

Overview

Key Topics and Technical Requirements

Applications

Who uses this standard

Related Standards

Технические детали

SIST EN ISO 5667-16:2017

Water quality - Sampling - Part 16: Guidance on biotesting of samples (ISO 5667-16:2017)

SIST EN ISO 5814:2013

Water quality - Determination of dissolved oxygen - Electrochemical probe method (ISO 5814:2012)

SIST-TS ISO/TS 20281:2010

Water quality - Guidance on statistical interpretation of ecotoxicity data