ISO 23065:2009 PDF

Name: ISO 23065:2009 — Milk fat from enriched dairy products — Determination of omega-3 and omega-6 fatty acid content by gas-liquid chromatography
SKU: ISO 23065:2009
Price: 25 EUR
Availability: InStock

Milk fat from enriched dairy products — Determination of omega-3 and omega-6 fatty acid content by gas-liquid chromatography

Название (английский):

ISO 23065:2009

Milk fat from enriched dairy products — Determination of omega-3 and omega-6 fatty acid content by gas-liquid chromatography

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 12
Дата публикации:: 26 февраля 2009 г.
Издание:: ISO IS 23065 edition 1 version 1
ICS:: 67.100.10

Тип поставки:

Full version180 €Read online 24h10 €Read online 72h15 €

Язык документа:

ruen

Срок поставки:2 раб. дн. (перевод)

Цена:180 €

Описание (английский):

ISO 23065:2009 specifies a method for the determination of the omega-3 and omega-6 fatty acid content in anhydrous milk fat extracted from dairy products supplemented or naturally enriched with these constituents. The specified procedure allows the evaluation of the most important omega-3 and omega-6 fatty acids.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 23065:2009 (IDF 211:2009) defines a standardized laboratory method for the determination of omega‑3 (ω−3) and omega‑6 (ω−6) fatty acid content in anhydrous milk fat extracted from dairy products that are supplemented or naturally enriched. The method uses preparation of fatty acid methyl esters (FAMEs) and separation/quantification by gas‑liquid chromatography (GLC / GC) with an internal standard. Key analytes include linoleic acid (LA), α‑linolenic acid (α‑LNA), EPA, DPA and DHA.

Key Topics

Scope and purpose: Quantitative determination of the most important ω−3 and ω−6 fatty acids in milk fat from enriched dairy products.

Principle: Addition of an internal standard (C23:0), transesterification of lipids to FAMEs, and separation by capillary GC with flame ionization detection (FID).

Reagents and standards: n‑hexane, high‑purity FAME standards (C23:0 internal standard, EPA, DHA, LA and others), and calibrated standard solutions.

Apparatus: Analytical balance, volumetric glassware, GC with capillary column and FID, high‑purity carrier gas (helium or hydrogen), split or on‑column injection and computerized integration.

Chromatographic considerations: Recommended capillary columns (polyethylene glycol or cyanosilicone phases), example conditions provided for helium isothermal or hydrogen temperature‑programmed runs to ensure separation (e.g., separation of C18:4ω−3 from conjugated linoleic acid and DPA from DHA).

Procedure overview: Extraction of anhydrous milk fat (ISO 14156/IDF 172), methylation (ISO 15884/IDF 182), calibration with EPA/DHA/LA and internal standard, qualitative and quantitative GC analysis, and calculation of mass fraction expressed in mg per 100 g fat.

Precision and reporting: Includes sections on repeatability, reproducibility and required content for the test report.

Applications and Users

Who uses it: Dairy laboratories, food testing laboratories, quality control teams in dairy and infant formula manufacturing, regulatory agencies and certification bodies assessing nutritional labeling claims for omega‑3/omega‑6 content.

Practical uses: Verifying enrichment claims, product development (fortified milks, yogurts, spreads), compliance testing for nutrition labeling, and research on fatty acid profiles in dairy products.

Related Standards

ISO 14156 / IDF 172 - Lipid extraction from milk and milk products

ISO 15884 / IDF 182 - Preparation of fatty acid methyl esters (FAMEs)

ISO 707 / IDF 50 - Recommended sampling of milk (sampling is not part of ISO 23065)

Keywords: ISO 23065:2009, milk fat, omega‑3, omega‑6, gas‑liquid chromatography, FAME, EPA, DHA, linoleic acid, anhydrous milk fat, dairy analysis.

ISO 23065:2009 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications and Users

Related Standards

Технические детали

ISO 14156:2001/Amd 1:2007

Milk and milk products — Extraction methods for lipids and liposoluble compounds — Amendment 1

BS ISO 15884:2002

Milk fat. Preparation of fatty acid methyl esters.

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)

BS ISO 23065:2009

Milk fat from enriched dairy products. Determination of omega-3 and omega-6 fatty acid content by gas-liquid…