SIST EN ISO 23753-1:2019 PDF

Name: SIST EN ISO 23753-1:2019 — Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC) (ISO 23753-1:2019)
SKU: SIST EN ISO 23753-1:2019
Price: 65.56 EUR
Availability: InStock

Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC) (ISO 23753-1:2019)

Название (английский):

SIST EN ISO 23753-1:2019

Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC) (ISO 23753-1:2019)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 17
Дата публикации:: 6 июня 2019 г.
ICS:: 13.080.30
Технический комитет:: KAT - Soil quality

Тип поставки:

Full version255 €Read online 24h10 €Read online 72h15 €

Язык документа:

ruen

Срок поставки:3 раб. дн. (перевод)

Цена:255 €

Описание (английский):

This document specifies a method for determining the activity of dehydrogenases enzymes in soil using 2,3,5-triphenyltetrazolium chloride (TTC).

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

EN ISO 23753-1:2019 (CEN adoption of ISO 23753-1:2019) specifies a standardized laboratory method for measuring dehydrogenase activity in soils using 2,3,5‑triphenyltetrazolium chloride (TTC). Dehydrogenases are intracellular microbial enzymes linked to respiratory activity; their activity is used as an indicator of viable, physiologically active soil microbial communities and overall soil quality.

Key topics and technical requirements

Principle: TTC is added to a soil sample; microbially mediated reduction produces triphenylformazan (TPF), which is extracted with ethanol and quantified by spectrophotometry at 485 nm.

Core conditions:

Incubation at 25 °C ± 1 °C for 6 h.
Substrate (TTC) prepared in Tris buffer (100 mmol/l, pH 7.6); recommended TTC concentration in the procedure is noted (e.g., 300 mmol/l preparation, 10%).
Extraction solvent: analytical grade ethanol (96 %) is specified; other solvents (e.g., acetone) are noted as alternatives.

Reagents and calibration:

Preparation of Tris buffer (100 mmol/l, pH 7.6), TTC substrate solution, and TPF stock/working solutions for standard curves.
Establishment of a TPF standard curve for quantification and validity checks.

Sampling and handling:

Follow ISO 18400‑206 for soil collection and handling.
If sieved fresh samples cannot be analysed immediately, storage up to 7 days at 4 °C ± 2 °C is acceptable.
Dry matter determination per ISO 11465.

Limitations and validity:

Abiotic reducers (e.g., Fe(II), sulfides), coal or carbonaceous materials, or very organic-rich substrates can bias results; mitigation checks (spiking with TPF and recovery) are recommended.
Storage time affects enzyme activity - samples with different storage times should not be compared.

Applications and users

This TTC-based soil enzyme assay is used for:

Environmental and agricultural soil quality monitoring

Research on soil microbial activity, biogeochemical cycles and soil management impacts

Assessing effects of contamination, remediation and land-use changes Primary users include environmental laboratories, academic researchers, agronomists, soil ecologists, and regulatory agencies involved in soil health and ecosystem assessments.

Related standards

ISO 11465 - Determination of dry matter and water content (gravimetric)

ISO 18400‑206 - Sampling: collection, handling and storage for microbiological assessment

EN ISO 23753‑1:2019 supersedes the previous edition (EN ISO 23753‑1:2011)

Keywords: soil quality, dehydrogenase activity, TTC method, triphenyltetrazolium chloride, soil enzyme assay, ISO 23753-1, microbial activity assay.

SIST EN ISO 23753-1:2019 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards

Технические детали

ISO 23753-1:2019

Soil quality — Determination of dehydrogenases activity in soils — Part 1: Method using triphenyltetrazolium…

SIST ISO 18400-206:2019

Soil quality -- Sampling -- Part 206: Collection, handling and storage of soil under aerobic conditions for t…

SIST EN ISO 11465:2026

Sludge and solid environmental matrices - Determination of dry residue or water content and calculation of th…

ISO 23753-1:2019/Amd 1:2020

Soil quality — Determination of dehydrogenases activity in soils — Part 1: Method using triphenyltetrazolium…