ISO 20976-2:2022

Name: ISO 20976-2:2022 — Microbiology of the food chain — Requirements and guidelines for conducting challenge tests of food and feed products — Part 2: Challenge tests to study inactivation potential and kinetic parameters
SKU: ISO 20976-2:2022
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Microbiology of the food chain — Requirements and guidelines for conducting challenge tests of food and feed products — Part 2: Challenge tests to study inactivation potential and kinetic parameters

Название (английский):

ISO 20976-2:2022

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 24
Дата публикации:: 6 октября 2022 г.
Издание:: ISO IS 20976 edition 1 version 1
ICS:: 07.100.30

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Описание (английский):

This document specifies the protocols for conducting microbiological challenge tests for inactivation studies on vegetative bacteria and bacterial spores in the raw materials and ingredients, intermediate or end products. The use of this document can be extended to yeasts which do not form mycelium.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 20976-2:2022 - Microbiology of the food chain - Requirements and guidelines for conducting challenge tests of food and feed products - Part 2 provides standardized protocols for microbiological challenge tests focused on inactivation. The standard covers methods to study the inactivation potential and inactivation kinetics of vegetative bacteria and bacterial spores in raw materials, ingredients, intermediate and end products. Its scope can be extended to non‑mycelial yeasts. ISO 20976-2:2022 supports validated control measures within HACCP, microbial risk assessment (MRA) and regulatory compliance.

Key topics and technical requirements

Scope and purpose: Defines when and how to conduct inactivation challenge tests and how they differ from growth studies (see ISO 20976-1).

Experimental design and sampling: Guidance on selecting batches, setting target reduction levels, number of units and sampling plans.

Apparatus, media and reagents: References to required laboratory equipment and culture media standards.

Selection and preparation of strains: Criteria for choosing challenge strains (including spore-formers) and standardized inoculum preparation.

Inoculation procedures and controls: Techniques for inoculating test units and requirements for uninoculated and inoculated controls.

Treatment and analysis: Protocols for applying inactivation treatments, enumeration methods, data collection and statistical analysis.

Kinetic parameters and metrics: Definitions and reporting of D value (decimal reduction time) and δ value (first decimal reduction time) and approaches for deriving primary and secondary inactivation parameters.

Simulation and modelling: Use of experimental data to simulate inactivation under varied conditions, supporting predictive modelling.

Reporting requirements: Detailed test report elements including aim, protocol, analytical results, conclusions and reference documents.

Normative references and annexes: Cross-references to ISO 6887, ISO 7218, ISO 11133 and informative/normative annexes with practical examples and minimum unit numbers.

Practical applications and users

ISO 20976-2:2022 is intended for:

Food business operators (FBOs) validating HACCP control measures and process lethality.

Food microbiology and quality laboratories performing challenge tests and process validations.

Regulators and auditors assessing compliance with food safety criteria.

Researchers and risk assessors conducting microbial risk assessment (MRA) and predictive modelling.

Manufacturers optimizing thermal processing, non‑thermal decontamination, storage and shelf‑life decisions.

Typical uses include validating kill‑step performance, determining D and δ values for pathogens/spoilers, comparing processing scenarios, and generating data for predictive inactivation models.

Related standards

ISO 20976-1:2019 - Challenge tests to study growth potential, lag time and maximum growth rate.

ISO 6887 (all parts) - Preparation of test samples and dilutions.

ISO 7218 - General requirements for microbiological examinations.

ISO 11133 - Preparation and performance testing of culture media.

Keywords: ISO 20976-2:2022, challenge tests, inactivation kinetics, D value, δ value, food safety, HACCP, bacterial spores, vegetative bacteria, microbiology of the food chain.

ISO 20976-2:2022

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Practical applications and users

Related standards

Технические детали

ISO 20976-1:2019

Microbiology of the food chain — Requirements and guidelines for conducting challenge tests of food and feed…

SIST EN ISO 20976-1:2019

Microbiology of the food chain - Requirements and guidelines for conducting challenge tests of food and feed…

ISO 6887-3:2017/Amd 1:2020

Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for mi…

SIST EN ISO 7218:2024

Microbiology of the food chain - General requirements and guidance for microbiological examinations (ISO 7218…

ISO 11133:2014/Amd 2:2020

Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of cul…